80% plant protein Manufacturers china using charge difference separation and purification knowledge
Protein molecules contain dissociable groups such as carboxyl and amino groups. Due to the different structures of different protein molecules, the composition of their ionizing groups and the exposure on the surface of the molecule are different, so the nature and net charge of different protein molecules are charged. Also different. According to the charge difference of protein molecules, electrophoresis and ion exchange chromatography can be used for separation and purification. Follow the editor of 80% plant protein Manufacturers china to have a simple understanding!
1. Ion exchange chromatography
Ion exchange chromatography is a technique for separating charged molecules such as proteins, peptides, and amino acids with different charged ions based on the difference in charge. When the pH of the solution is greater than the isoelectric point of the protein, the protein molecules are negatively charged and can bind to the anion exchanger; when the pH of the solution is less than the isoelectric point of the protein, the protein molecules are positively charged and can bind to cations On the exchanger.
In a solution with a certain pH value, protein molecules can be dissociated into positively and negatively charged molecules. In a DC electric field, positively charged molecules move to the negative electrode, and negatively charged molecules move to the positive electrode. Different protein molecules are separated from each other due to their different net charge and molecular weight. Common electrophoresis includes polyacrylamide gel electrophoresis, two-dimensional electrophoresis, isoelectric focusing electrophoresis and so on.
In summary, the editor of 80% plant protein Manufacturers china introduces: knowledge about the separation and purification of plant protein using charge difference.