Do you know what the separation and purification method of customized plant protein For sale is?
Customized plant protein For sale uses the difference of solubility to separate and purify the method, do you know what it is? Let’s take a look at it with the editor below!
Different customized plant protein For sale have different solubility. The solubility of protein molecules in water mainly depends on the thickness of the hydration layer on the surface and the number of charges. Different protein molecules have different types, numbers and arrangements of polar groups on their molecular surfaces, and their hydration layer thickness and number of charges are also different, resulting in different solubility of proteins. In addition, external environmental factors such as the pH value, ionic strength, temperature and dielectric constant of the solution can all affect its solubility.
1. Salting out method
The salting-out method is to add a high concentration of neutral salt to the customized plant protein For sale solution. The interaction between the salt ion and the water molecule can reduce the activity of the water molecule and its degree of hydration, thereby reducing the solubility of the protein and forming a precipitate. The process of precipitation. The commonly used salting-out agent is ammonium sulfate, because of its strong salting-out ability, and high concentration will not lose the biological activity of the customized plant protein For sale. The advantage of the salting-out method is that it is easy to operate, can remove more impurity proteins, can protect fragile proteins, and has a certain concentration effect.
2. Isoelectric point precipitation method
The principle of the isoelectric point precipitation method: The customized plant protein For sale is an amphoteric electrolyte, and its solubility is related to the amount of net charge. When the pH of the solution is equal to the isoelectric point of the protein, its solubility is very low, forming a precipitate. Different customized plant protein For sale have different isoelectric points. Using the difference of protein isoelectric point, adjust the pH value of the solution to be equal to the isoelectric point of the target protein, so that the target protein can be precipitated, and the desired target protein can be obtained by centrifugation.
The above is the editor's description: About the introduction of the separation and purification method of customized plant protein For sale using the difference of solubility, I hope you can learn more.